Periodontal Bacterial DNA and Their Link to Human Cardiac Tissue: Findings of a Pilot Study

Bacteria from the mouth DO travel to distant sites of the body which is the definition of focal infection. Foci of infection in the mouth can infect other organs in the body which may cause or contribute to disease. 

Dr. Cole Sommers

Thorac Cardiovasc Surg. 2015 Oct 5. [Epub ahead of print]

Periodontal Bacterial DNA and Their Link to Human Cardiac Tissue: Findings of a Pilot Study.

Ziebolz D1, Rost C2, Schmidt J2, Waldmann-Beushausen R3, Schöndube FA3, Mausberg RF2, Danner BC3.

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The aim of this pilot study was to detect correlations of microbiological DNA, inflammatory proteins, and infection parameters in patients with periodontal disease (PD) and valvular heart disease (VHD). 


A perioperative comprehensive dental examination for the investigation of periodontal status, including sampling of specific subgingival bacteria, was performed in 10 patients with indication for surgery of aortic valve stenosis with or without concomitant myocardial revascularization. Standard protocol biopsies were taken from right atrium (A), left septal myocardium (M), and aortic valve (V). Eleven periodontal pathogens DNA in oral and cardiac tissue samples (A/M/V) were analyzed using polymerase chain reaction. For cardiac tissue samples, Western blot analysis of LPS-binding protein (LBP), immunohistochemical (IHC) detection of LBP-big42, LPS-binding protein receptor (CD14), and macrophages (CD68), as well as inflammation scoring measurement were performed. 


Periodontitis was present in all patients with severe intensity in 7, moderate in 2 and mild in one patient. Same bacterial DNA was detected in A, M, and V in different distribution, and detection was more often in atrium than in myocardium or valve tissue. Morphological investigation revealed increased extracellular inflammatory cell migration. In IHC markers of LBP, CD68 and CD14 showed positive findings for all patients in atrium and myocardium. 


Our results demonstrate the presence of oral bacterial DNA in human cardiac tissue, as well as inflammatory markers potentially indicating connection of PD and VHD. Further investigation is necessary to confirm these preliminary data.

Georg Thieme Verlag KG Stuttgart · New York.

PMID: 26441242 [PubMed – as supplied by publisher]

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